XXI International Congress of Theoretical and Applied Mechanics
Warsaw, Poland, August 15-21, 2004

In Vivo PIV Measurement in the Embryonic Chicken Heart

Peter Vennemann (1), Kenneth T. Kiger (2), Beerend P. Hierck (3), Nicolette T. C. Ursem (4), Timo L. M. Ten Hagen (5), Jerry Westerweel (1)
1. Laboratory for Aero- and Hydrodynamics, Delft Technical Univ, the Netherlands
2. Department of Mechanical Engineering, University of Maryland, USA
3. Department of Anatomy and Embryology, Leiden University MC,the Netherlands
4. Department of Obstetrics and Gynaecology, Erasmus MC, the Netherlands
5. Department of Surgical Oncology, Erasmus MC, Netherlands

Blood flow is an important factor influencing the embryonic heart development. The flow induces shear stress that act on the vascular endothelium and modulate gene expression. Previous work has qualitatively shown that modification of the flow through the heart can significantly alter the heart development. To enlighten the relationship between fluid shear stress and gene expression, the blood flow of a chicken embryo is manipulated. Combining the fluorescent visualization of gene expression with a quantitative measurement of the instantaneous flow field using PIV, a direct relation between shear stress and gene response can be found. In vivo PIV measurements are performed at different phase angles of the cardiac cycle. Fluorescent lipid micro-spheres serve as tracer particles. The velocity distribution within the ventricle and the atrium is resolved. Current work is focused on improving the spatial resolution and accuracy in the near-wall region along with accurate registration of the wall boundary.

Extended Summary (PDF)